High magnetic field induced changes of gene expression in arabidopsis.

ABSTRACT:

Background

High magnetic fields are becoming increasingly prevalent components of non-invasive, biomedical imaging tools (such as MRI), thus, an understanding of the molecular impacts associated with these field strengths in biological systems is of central importance. The biological impact of magnetic field strengths up to 30 Tesla were investigated in this study through the use of transgenic Arabidopsis plants engineered with a stress response gene consisting of the alcohol dehydrogenase (Adh) gene promoter driving the beta-glucuronidase (GUS) gene reporter.

Methods

Magnetic field induced Adh/GUS activity was evaluated with histochemical staining to assess tissue specific expression and distribution, and with quantitative, spectrofluometric assays to measure degree of acti

SUBMITTER: Paul AL 

PROVIDER: S-EPMC1764872 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature