A PDZ domain recapitulates a unifying mechanism for protein folding.

ABSTRACT: A unifying view has been recently proposed according to which the classical diffusion-collision and nucleation-condensation models may represent two extreme manifestations of an underlying common mechanism for the folding of small globular proteins. We report here the characterization of the folding process of the PDZ domain, a protein that recapitulates the three canonical steps involved in this unifying mechanism, namely: (i) the early formation of a weak nucleus that determines the native-like topology of a large portion of the structure, (ii) a global collapse of the entire polypeptide chain, and (iii) the consolidation of the remaining partially structured regions to achieve the native state conformation. These steps, which are clearly detectable in the PDZ domain investigated here, m

SUBMITTER: Gianni S 

PROVIDER: S-EPMC1765422 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature