Dataset Information
Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis.
Ontology highlight
ABSTRACT: Background
Opiate addiction reflects plastic changes that endurably alter synaptic transmission within relevant neuronal circuits. The biochemical mechanisms of these adaptations remain largely unknown and proteomics-based approaches could lead to a broad characterization of the molecular events underlying adaptations to chronic drug exposure.Results
Thus, we have started proteomic analyses of the effects of chronic morphine exposure in a recombinant human neuroblastoma SH-SY5Y clone that stably overexpresses the mu-opioid receptor. Cells were treated with morphine for 6, 24 and 72 hours, the proteins were separated by 2-D gel electrophoresis and stained with Coomassie blue, and the protein map was compared with that obtained from untreated cells. Spots showing a statistically significant variation were selected for identification using mass spectrometric analyses.Conclusion
A total of 45 proteins were identified, including proteins involved in cellular metabolism, cytoskeleton organization, vesicular trafficking, transcriptional and translational regulation, and cell signaling.
SUBMITTER: Neasta J
PROVIDER: S-EPMC1766345 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Publications
Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis.
Proteome science 20061221
<h4>Background</h4>Opiate addiction reflects plastic changes that endurably alter synaptic transmission within relevant neuronal circuits. The biochemical mechanisms of these adaptations remain largely unknown and proteomics-based approaches could lead to a broad characterization of the molecular events underlying adaptations to chronic drug exposure.<h4>Results</h4>Thus, we have started proteomic analyses of the effects of chronic morphine exposure in a recombinant human neuroblastoma SH-SY5Y c ...[more]