Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis.

ABSTRACT:

Background

Opiate addiction reflects plastic changes that endurably alter synaptic transmission within relevant neuronal circuits. The biochemical mechanisms of these adaptations remain largely unknown and proteomics-based approaches could lead to a broad characterization of the molecular events underlying adaptations to chronic drug exposure.

Results

Thus, we have started proteomic analyses of the effects of chronic morphine exposure in a recombinant human neuroblastoma SH-SY5Y clone that stably overexpresses the mu-opioid receptor. Cells were treated with morphine for 6, 24 and 72 hours, the proteins were separated by 2-D gel electrophoresis and stained with Coomassie blue, and the protein map was compared with that obtained from untreated cells. Spots showing a statistica

SUBMITTER: Neasta J 

PROVIDER: S-EPMC1766345 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature