Multiplexed expression and screening for recombinant protein production in mammalian cells.

ABSTRACT:

Background

A variety of approaches to understanding protein structure and function require production of recombinant protein. Mammalian based expression systems have advantages over bacterial systems for certain classes of protein but can be slower and more laborious. Thus the availability of a simple system for production and rapid screening of constructs or conditions for mammalian expression would be of great benefit. To this end we have coupled an efficient recombinant protein production system based on transient transfection in HEK-293 EBNA1 (HEK-293E) suspension cells with a dot blot method allowing pre-screening of proteins expressed in cells in a high throughput manner.

Results

A nested PCR approach was used to clone 21 extracellular domains of mouse receptors as CD4

SUBMITTER: Chapple SD 

PROVIDER: S-EPMC1769369 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature