Proteomic profiling of the amniotic fluid to detect inflammation, infection, and neonatal sepsis.

ABSTRACT:

Background

Proteomic analysis of amniotic fluid shows the presence of biomarkers characteristic of intrauterine inflammation. We sought to validate prospectively the clinical utility of one such proteomic profile, the Mass Restricted (MR) score.

Methods and findings

We enrolled 169 consecutive women with singleton pregnancies admitted with preterm labor or preterm premature rupture of membranes. All women had a clinically indicated amniocentesis to rule out intra-amniotic infection. A proteomic fingerprint (MR score) was generated from fresh samples of amniotic fluid using surface-enhanced laser desorption ionization (SELDI) mass spectrometry. Presence or absence of the biomarkers of the MR score was interpreted in relationship to the amniocentesis-to-delivery interval, plac

SUBMITTER: Buhimschi CS 

PROVIDER: S-EPMC1769412 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature