ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis.

ABSTRACT:

Background

ICI 182,780 (ICI) belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in addition to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (ER) actions in several mammalian species. In the present study, the effects and mechanism of action of ICI were investigated in the teleost fish, sea bream (Sparus auratus).

Methods

Three independent in vivo experiments were performed in which mature male tilapia (Oreochromis mossambicus) or sea bream received intra-peritoneal implants containing estradiol-17 beta (E2), ICI or a combination of both compounds. The effects of E2 and ICI on plasma calcium levels were measured and hepatic and testicular gene expression of the three ER subtypes, ER alpha, ER beta a and

SUBMITTER: Pinto PI 

PROVIDER: S-EPMC1769500 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature