A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits.

ABSTRACT:

Aims

Tissue transglutaminase (tTG) is a major autoantigen recognised by IgA anti-endomysial antibodies (IgA EMA). Enzyme linked immunosorbent assays (ELISA) for IgA anti-tissue transglutaminase antibodies (IgA tTG) have therefore been developed as an alternative serological screening test to IgA EMA for coeliac disease (CD). The use of human tTG (h-tTG), as opposed to guinea pig liver tTG (gpl-tTG), in these assays has been reported to produce superior results. This study compared 13 commercial IgA tTG ELISA kits to ascertain their performance characteristics in the diagnosis of CD in patients with biopsy confirmed disease compared with controls. All patients and controls were adults aged 21 years or older.

Methods

Sera from the following groups of patients were tested in ea

SUBMITTER: Wong RC 

PROVIDER: S-EPMC1769686 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature