Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing.

ABSTRACT: AIMS:To define epidemiology, clinical disease, and outcome of gemella bacteraemia by 16S rRNA gene sequencing. To examine the usefulness of the Vitek, API, and ATB systems in identifying two gemella species. METHODS:All alpha haemolytic streptococci other than Streptococcus pneumoniae isolated from blood cultures during a six year period were identified by conventional biochemical methods, the Vitek system, and the API system. 16S rRNA gene sequencing was performed on all isolates identified by both kits as gemella with >or= 95% confidence or by either kit as any bacterial species with < 95% confidence. The ATB expression system was used to identify the two isolates that were defined as gemella species by 16S rRNA gene sequencing. RESULTS:Of the 302 alpha haemolytic streptococci other than

SUBMITTER: Woo PC 

PROVIDER: S-EPMC1770062 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature