High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides.

ABSTRACT:

Background

Currently, comparative genomic hybridisation array (array CGH) is the method of choice for studying genome wide DNA copy number changes. To date, either amplified representations of bacterial artificial chromosomes (BACs)/phage artificial chromosomes (PACs) or cDNAs have been spotted as probes. The production of BAC/PAC and cDNA arrays is time consuming and expensive.

Aim

To evaluate the use of spotted 60 mer oligonucleotides (oligos) for array CGH.

Methods

The hybridisation of tumour cell lines with known chromosomal aberrations on to either BAC or oligoarrrays that are mapped to the human genome.

Results

Oligo CGH was able to detect amplifications with high accuracy and greater spatial resolution than other currently used array CGH platforms. In ad

SUBMITTER: Carvalho B 

PROVIDER: S-EPMC1770328 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature