Aberrations in the mismatch repair genes and the clinical impact on oesophageal squamous carcinomas from a high incidence area in South Africa.

ABSTRACT:

Aims

To investigate the incidence of genetic aberrations in the DNA repair genes in a cohort of oesophageal cancers.

Methods

One hundred oesophagectomy samples of squamous cell carcinoma were studied. Normal and tumour DNA were isolated using a standard phenol/chloroform extraction procedure. Six recommended microsatellite loci with high informativity were analysed. The following markers were used: D2S123 (2p), D3S659 (3p), D3S1255 (3p), Bat 25 (4q), Bat 26 (2p), and Bat 40 (1p). The results were analysed using software attached to an automated DNA sequencer. The molecular data were then correlated with clinicopathological parameters.

Results

The incidence of microsatellite instability and loss of heterozygosity was very low. There was no significant correlation betwe

SUBMITTER: Naidoo R 

PROVIDER: S-EPMC1770598 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature