Aberrant expression of DeltaNp73 in benign and malignant tumours of the prostate: correlation with Gleason score.

ABSTRACT:

Background

The p73 gene is a p53 homologue that induces apoptosis and inhibits cell proliferation. N-terminal truncated isoforms of p73 (DeltaNp73) act as dominant-negative inhibitors of wild-type p53 and TAp73 and result in tumour growth in nude mice.

Aims

To detect DeltaNp73 expression in 24 benign prostatic hyperplasia samples, 33 prostate carcinomas, and five normal samples and to evaluate the relation between DeltaNp73, TAp73 concentrations, and the clinicopathological characteristics of patients with prostate cancer.

Methods

TAp73 was determined by real time polymerase chain reaction (PCR); DeltaNp73 and DeltaN'p73 were assessed using reverse transcription PCR. western blotting was used to analyse protein expression. p53 mutation was determined by immunohistoche

SUBMITTER: Guan M 

PROVIDER: S-EPMC1770779 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature