New approaches for genotyping paraffin wax embedded breast tissue from patients with cancer: the Iowa women's health study.

ABSTRACT:

Background

The use of paraffin wax embedded tissue samples as a source of DNA for genotype analysis has been limited because of difficulties in DNA extraction and single nucleotide polymorphism (SNP) analysis.

Aims

To test the feasibility of applying the combination of a commonly used DNA isolation procedure, PureGene, and a high throughput SNP analysis method, the polymerase chain reaction (PCR)-INVADER assay, to genotype several types of paraffin wax embedded breast tissues.

Methods

Twenty formalin fixed, paraffin wax blocks were obtained from five participants in the Iowa women's health study. Each participant provided several types of tissue including normal lymph node, normal nipple/areola tissue, inflammatory/fibrotic breast tissue, or normal breast tissue, and

SUBMITTER: Thyagarajan B 

PROVIDER: S-EPMC1770813 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature