Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium.

ABSTRACT:

Background

Renin-angiotensin system (RAS) components have been identified in human ciliary body and aqueous humour, pointing to a role for the RAS in the regulation of aqueous humour dynamics. Here, the authors examine the functional expression of a RAS and the effects of angiotensin II (AII) on a signal transduction pathway and ion secretion mechanism in cultured human ciliary body non-pigmented epithelium (HNPE).

Methods

RAS expression was examined in cultured HNPE cells using polymerase chain reaction (PCR) analysis. Secretory function was determined using spectrofluorescence imaging microscopy to measure cell calcium (Ca(2+)(I)) and volume responses. Single channel patch clamp techniques were employed to investigate ion channel activity.

Results

PCR analysis demon

SUBMITTER: Cullinane AB 

PROVIDER: S-EPMC1771149 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature