Lactoferrin Glu561Asp facilitates secondary amyloidosis in the cornea.

ABSTRACT:

Aim

To elucidate the pathogenic mechanism of amyloid formation in corneal amyloidosis with trichiasis.

Methods

Ophthalmological examination was performed in nine patients to determine secondary corneal amyloidosis with trichiasis. Congo red staining and immunohistochemistry using anti-human lactoferrin antibody were used for biopsied corneal samples. For genetic analyses, single strand conformation polymorphism (SSCP), direct DNA sequence analysis, and polymerase chain reaction (PCR) induced mutation restriction analysis (IMRA) were employed to detect lactoferrin gene polymorphism.

Results

All patients had had trichiasis at least for 1 year, and all amyloid-like deposits were found in one eye with trichiasis. Ophthalmological examination revealed that eight patients s

SUBMITTER: Araki-Sasaki K 

PROVIDER: S-EPMC1772658 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature