The expression of native and cultured human retinal pigment epithelial cells grown in different culture conditions.

ABSTRACT:

Aim

To determine the transcriptional proximity of retinal pigment epithelium (RPE) cells grown under different culture conditions and native RPE.

Methods

ARPE-19 cells were grown under five conditions in 10% CO(2): "subconfluent" in DMEM/F12+10% FBS, "confluent" in serum and serum withdrawn, and "differentiated" for 2.5 months in serum and serum withdrawn medium. Native RPE was laser microdissected. Total RNA was extracted, reverse transcribed, and radiolabelled probes were hybridised to an array containing 5,353 genes. Arrays were evaluated by hierarchical cluster analysis and significance analysis of microarrays.

Results

78% of genes were expressed by native RPE while 45.3--47.7% were expressed by ARPE-19 cells, depending on culture condition. While the most abundan

SUBMITTER: Tian J 

PROVIDER: S-EPMC1772910 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature