Non-genomic regulation of intermediate conductance potassium channels by aldosterone in human colonic crypt cells.

ABSTRACT: BACKGROUND:Aldosterone has a rapid, non-genomic, inhibitory effect on macroscopic basolateral K(+) conductance in the human colon, reducing its capacity for Cl(-) secretion. The molecular identity of the K(+) channels constituting this aldosterone inhibitable K(+) conductance is unclear. AIM:To characterise the K(+) channel inhibited by aldosterone present in the basolateral membrane of human colonic crypt cells. METHODS:Crypts were isolated from biopsies of healthy sigmoid colon obtained during colonoscopy. The effect of aldosterone on basolateral K(+) channels, and the possible involvement of Na(+):H(+) exchange, were studied by patch clamp techniques. Total RNA from isolated crypts was subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using primers specific to interm

SUBMITTER: Bowley KA 

PROVIDER: S-EPMC1773689 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature