Duodenal expression of iron transport molecules in untreated haemochromatosis subjects.

ABSTRACT: BACKGROUND AND AIMS:In HFE associated hereditary haemochromatosis, the duodenal enterocyte behaves as if iron deficient and previous reports have shown increased duodenal expression of divalent metal transporter 1 (DMT1) and iron regulated gene 1 (Ireg1) in affected subjects. In those studies, many patients had undergone venesection, which is a potent stimulus of iron absorption. Our study investigated duodenal expression of DMT1 (IRE and non-IRE), Ireg1, hephaestin, and duodenal cytochrome-b (Dyctb) in untreated C282Y homozygous haemochromatosis patients, iron deficient patients, and iron replete subjects. METHODS:Total RNA was extracted from duodenal biopsies and expression of the iron transport genes was assessed by ribonuclease protection assay. RESULTS:Expression of DMT1 (IRE) and Ire

SUBMITTER: Stuart KA 

PROVIDER: S-EPMC1773733 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature