Gene expression pattern of laser microdissected colonic crypts of adenomas with low grade dysplasia.

ABSTRACT: BACKGROUND AND AIMS:Colorectal epithelial cells are prone to malignant transformation. Therefore, identification of differences in gene expression in the process from normal colonic crypts to adenomas with low grade dysplasia is essential for further insights into early tumorigenesis. To achieve this goal, a novel gene expression analysis strategy, screening for expressed transcripts in small histologically defined tissue samples, was performed. METHODS:First, laser mediated microdissection was used to isolate normal and adenomatous crypts from colonic cryosections. Then, nested RNA arbitrarily primed polymerase chain reaction (RAP-PCR) for differential display was performed to screen mRNA populations and to generate hybridisation probes for cDNA expression arrays. After evaluation of cDNA

SUBMITTER: Lechner S 

PROVIDER: S-EPMC1773735 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature