Intestinal epithelial exosomes carry MHC class II/peptides able to inform the immune system in mice.

ABSTRACT: BACKGROUND:Intestinal epithelial cells secrete exosome-like vesicles. The aim of this study was to characterise murine intestinal epithelial exosomes and to analyse their capacity to inform the immune system in vivo in mice. METHODS:Epithelial exosomes were obtained from the murine epithelial cell line MODE K incubated in the presence or absence of interferon gamma (IFN-gamma) together with pepsin/trypsin ovalbumin hydrolysate (hOVA) to mimic luminal digestion. Exosomes isolated from MODE K conditioned media (EXO-hOVA and EXO-hOVA-IFN) were characterised by western blot, peptide mapping, and mass spectrometry. They were injected intraperitoneally to C3H/HeN mice to test their immunocompetence. RESULTS:MODE K epithelial exosomes displayed major histocompatibility complex (MHC) class I and c

SUBMITTER: Van Niel G 

PROVIDER: S-EPMC1773888 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature