Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction.

ABSTRACT:

Background

Desulfovibrios produce sulphide, which is toxic to colonic epithelial cells. These bacteria have previously been linked to ulcerative colitis. Traditional methods of culturing these organisms are slow, and often unreliable, while molecular approaches are either non-quantitative or lack sensitivity.

Aims

To develop a sensitive method for quantitating desulfovibrios in stools and biopsy tissue, and to investigate the effects of age and disease on these bacteria.

Methods

Rectal biopsies were taken from 10 colitis patients and 10 healthy controls. Stool samples were obtained from 10 healthy infants (mean age 1.01 (0.18) years), 10 healthy young adults (26.7 (1.2) years), and 10 healthy elderly people (71.7 (1.2) years). Primers were designed and developed for a

SUBMITTER: Fite A 

PROVIDER: S-EPMC1774019 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature