A microarray screen for novel candidate genes in coeliac disease pathogenesis.

ABSTRACT:

Background and aims

The causative molecular pathways underlying the pathogenesis of coeliac disease are poorly understood. To unravel novel aspects of disease pathogenesis, we used microarrays to determine changes in gene expression of duodenal biopsies.

Methods

cDNA microarrays representing 19 200 genes were used to compare gene expression profiles of duodenal biopsies from 15 coeliac disease patients with villous atrophy (Marsh III) and seven control individuals with normal biopsies (Marsh 0). In addition, the specific effect of gluten was studied by comparing the expression profiles of Marsh III lesions of seven patients exposed to gluten with four patients on a gluten free diet.

Results

Comparing Marsh III with Marsh 0 lesions identified 109 genes that differed si

SUBMITTER: Diosdado B 

PROVIDER: S-EPMC1774096 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature