IgA antibodies of coeliac disease patients recognise a dominant T cell epitope of A-gliadin.

ABSTRACT:

Background

In coeliac disease (CD) patients, the dominant DQ2-Alpha-I-gliadin peptide recognised by CD4 T cells is contained within peptide sequence 57-73 (p57-73) of Alpha-gliadin. This peptide sequence is also located within a 33-mer protease resistant gliadin fragment and therefore is likely to play an important role in the pathogenesis of CD.

Aims

Our aim was to determine whether a B cell epitope was present within the immunodominant T cell epitope of Alpha-gliadin and, if so, to elucidate its sequence and determine the importance of deamidation and/or modification of the amino acid at position 65 for IgA binding.

Patients and methods

A cohort of CD patients, disease controls, and healthy individuals were examined. Serum IgA antibodies to the native and modified p

SUBMITTER: Bateman EA 

PROVIDER: S-EPMC1774203 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature