Bile secretory function in the obese Zucker rat: evidence of cholestasis and altered canalicular transport function.

ABSTRACT:

Background

Obese Zucker rats (ZR) have been used as an experimental model for non-alcoholic fatty liver disease and are particularly susceptible to various types of liver injury. Bile secretory function has not been assessed in ZR.

Aim

To study bile secretion and expression of the main hepatobiliary transporters in ZR.

Methods

Bile flow and biliary secretion of lipids and glutathione were determined in eight and 14 week old obese ZR and their lean controls. Protein mass and mRNA of the Na(+)/taurocholate cotransporting polypeptide (Ntcp), the bile salt export pump (Bsep), and the multidrug resistant associated protein 2 (Mrp2) were assessed by western and northern blot, respectively. The effects of administration of a tumour necrosis factor alpha inactivator (etanerce

SUBMITTER: Pizarro M 

PROVIDER: S-EPMC1774316 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature