Use of microsatellite marker loss of heterozygosity in accurate diagnosis of pancreaticobiliary malignancy from brush cytology samples.

ABSTRACT:

Background

Brush cytology of biliary strictures to diagnose pancreaticobiliary malignancy suffers from poor sensitivity.

Aim

To improve the diagnostic yield of pancreaticobiliary brush cytology through analysis of tumour suppressor gene linked microsatellite marker loss of heterozygosity (LOH) and k-ras codon 12 mutation detection.

Methods

Twenty six patients with biliary strictures underwent endoscopic retrograde cholangiography with brush cytology. A panel of 12 polymorphic microsatellite markers linked to six tumour suppressor genes was developed. Genomic DNA from cell clusters acquired from brush cytology specimens and microdissected surgical malignant and normal tissue underwent polymerase chain amplification reaction (PCR). PCR products were compared for LOH and

SUBMITTER: Khalid A 

PROVIDER: S-EPMC1774321 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature