CD40 antisense oligonucleotide inhibition of trinitrobenzene sulphonic acid induced rat colitis.

ABSTRACT:

Background

CD154/CD40 interactions play a pivotal role both in humoral and cellular immune responses. Their involvement in the pathogenesis of chronic inflammatory bowel disease (IBD) has been revealed by increased expression of CD40 and CD154 in the inflamed mucosa of patients and the therapeutic effects of anti-CD154 antibodies in experimental colitis. Because of adverse side effects however, the use of such antibodies in patients with IBD may be limited.

Aims

An alternative approach to blocking CD154/CD40 interactions by employing a CD40 antisense oligonucleotide (ODN) was explored.

Results

After sequencing of the rat CD40 gene, five antisense ODNs were designed, of which one (rAS3) effectively downregulated CD40 expression in rat vascular smooth muscle cells as we

SUBMITTER: Gao D 

PROVIDER: S-EPMC1774378 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature