Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri.

ABSTRACT:

Background

The multicellular alga Volvox carteri possesses only two cell types: mortal, motile somatic cells and potentially immortal, immotile reproductive cells. It is therefore an attractive model system for studying how cell-autonomous cytodifferentiation is programmed within a genome. Moreover, there are ongoing genome projects both in Volvox carteri and in the closely related unicellular alga Chlamydomonas reinhardtii. However, gene sequencing is only the beginning. To identify cell-type specific expression and to determine relative expression rates, we evaluate the potential of real-time RT-PCR for quantifying gene transcript levels.

Results

Here we analyze a diversified pool of 39 target genes by real-time RT-PCR for each cell type. This gene pool contains previously

SUBMITTER: Nematollahi G 

PROVIDER: S-EPMC1774577 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature