T cells in peripheral blood after gluten challenge in coeliac disease.

ABSTRACT:

Background

Current understanding of T cell epitopes in coeliac disease (CD) largely derives from intestinal T cell clones in vitro. T cell clones allow identification of gluten peptides that stimulate T cells but do not quantify their contribution to the overall gluten specific T cell response in individuals with CD when exposed to gluten in vivo.

Aims

To determine the contribution of a putative dominant T cell epitope to the overall gliadin T cell response in HLA-DQ2 CD in vivo.

Patients

HLA-DQ2+ individuals with CD and healthy controls.

Methods

Subjects consumed 20 g of gluten daily for three days. Interferon gamma (IFN-gamma) ELISPOT was performed using peripheral blood mononuclear cells (PBMC) to enumerate and characterise peptide and gliadin specific T cel

SUBMITTER: Anderson RP 

PROVIDER: S-EPMC1774637 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature