Serum antibodies to carbonic anhydrase IV in patients with autoimmune pancreatitis.

ABSTRACT:

Background and aims

Serum antibodies to carbonic anhydrase (CA) II have been reported in patients with autoimmune pancreatitis (AIP) and Sjogren's syndrome (SjS). However, their significance in the pathogenesis of these diseases is controversial. The aim of this study was to identify serum antibodies to CA isozymes, which are expressed in ductal cells of the pancreas.

Methods

Recombinant proteins of human CAs IV, IX, and XII were obtained using a bacterial expression system, and five CA IV peptides with theoretically high antigenicity were synthesised. Western blotting and enzyme linked immunosorbent assay (ELISA) were used to detect serum antibodies to the CA isozymes.

Results

The first screening analysis by western blot showed serum antibodies to CA IV among three C

SUBMITTER: Nishimori I 

PROVIDER: S-EPMC1774835 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature