Large scaled analysis of hepatitis B virus (HBV) DNA integration in HBV related hepatocellular carcinomas.

ABSTRACT:

Background and aims

Hepatitis B virus (HBV) DNA integration into or close to cellular genes is frequently detected in HBV positive hepatocellular carcinomas (HCC). We have previously shown that viral integration can lead to aberrant target gene transcription. In this study, we attempted to investigate common pathways to hepatocarcinogenesis.

Methods

By using a modified Alu-polymerase chain reaction approach, we analysed 50 HCCs along with 10 previously published cases.

Results

Sixty eight cellular flanking sequences (seven repetitive or unidentified sequences, 42 cellular genes, and 19 sequences potentially coding for unknown proteins) were obtained. Fifteen cancer related genes and 25 cellular genes were identified. HBV integration recurrently targeted the human telo

SUBMITTER: Murakami Y 

PROVIDER: S-EPMC1774867 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature