Distinctive gene expression patterns in human mammary epithelial cells and breast cancers.

ABSTRACT: cDNA microarrays and a clustering algorithm were used to identify patterns of gene expression in human mammary epithelial cells growing in culture and in primary human breast tumors. Clusters of coexpressed genes identified through manipulations of mammary epithelial cells in vitro also showed consistent patterns of variation in expression among breast tumor samples. By using immunohistochemistry with antibodies against proteins encoded by a particular gene in a cluster, the identity of the cell type within the tumor specimen that contributed the observed gene expression pattern could be determined. Clusters of genes with coherent expression patterns in cultured cells and in the breast tumors samples could be related to specific features of biological variation among the samples. Two such

SUBMITTER: Perou CM 

PROVIDER: S-EPMC17759 | biostudies-literature | 1999 Aug

REPOSITORIES: biostudies-literature