Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data.

ABSTRACT:

Introduction

Diverse microarray and sequencing technologies have been widely used to characterise the molecular changes in malignant epithelial cells in breast cancers. Such gene expression studies to identify markers and targets in tumour cells are, however, compromised by the cellular heterogeneity of solid breast tumours and by the lack of appropriate counterparts representing normal breast epithelial cells.

Methods

Malignant neoplastic epithelial cells from primary breast cancers and luminal and myoepithelial cells isolated from normal human breast tissue were isolated by immunomagnetic separation methods. Pools of RNA from highly enriched preparations of these cell types were subjected to expression profiling using massively parallel signature sequencing (MPSS) and four

SUBMITTER: Grigoriadis A 

PROVIDER: S-EPMC1779497 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature