Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system.

ABSTRACT:

Background

Mass spectrometry has become a powerful tool for the analysis of large numbers of proteins in complex samples, enabling much of proteomics. Due to various analytical challenges, so far no proteome has been sequenced completely. O'Shea, Weissman and co-workers have recently determined the copy number of yeast proteins, making this proteome an excellent model system to study factors affecting coverage.

Results

To probe the yeast proteome in depth and determine factors currently preventing complete analysis, we grew yeast cells, extracted proteins and separated them by one-dimensional gel electrophoresis. Peptides resulting from trypsin digestion were analyzed by liquid chromatography mass spectrometry on a linear ion trap-Fourier transform mass spectrometer with ver

SUBMITTER: de Godoy LM 

PROVIDER: S-EPMC1779535 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature