Analysis of gene expression in operons of Streptomyces coelicolor.

ABSTRACT:

Background

Recent studies have shown that microarray-derived gene-expression data are useful for operon prediction. However, it is apparent that genes within an operon do not conform to the simple notion that they have equal levels of expression.

Results

To investigate the relative transcript levels of intra-operonic genes, we have used a Z-score approach to normalize the expression levels of all genes within an operon to expression of the first gene of that operon. Here we demonstrate that there is a general downward trend in expression from the first to the last gene in Streptomyces coelicolor operons, in contrast to what we observe in Escherichia coli. Combining transcription-factor binding-site prediction with the identification of operonic genes that exhibited higher tr

SUBMITTER: Laing E 

PROVIDER: S-EPMC1779546 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature