Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

ABSTRACT:

Background

The tear film is a thin layer of fluid that covers the ocular surface and is involved in lubrication and protection of the eye. Little is known about the protein composition of tear fluid but its deregulation is associated with disease states, such as diabetic dry eyes. This makes this body fluid an interesting candidate for in-depth proteomic analysis.

Results

In this study, we employ state-of-the-art mass spectrometric identification, using both a hybrid linear ion trap-Fourier transform (LTQ-FT) and a linear ion trap-Orbitrap (LTQ-Orbitrap) mass spectrometer, and high confidence identification by two consecutive stages of peptide fragmentation (MS/MS/MS or MS3), to characterize the protein content of the tear fluid. Low microliter amounts of tear fluid samples

SUBMITTER: de Souza GA 

PROVIDER: S-EPMC1779605 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature