Identification of putative cis-regulatory elements in Cryptosporidium parvum by de novo pattern finding.

ABSTRACT:

Background

Cryptosporidium parvum is a unicellular eukaryote in the phylum Apicomplexa. It is an obligate intracellular parasite that causes diarrhea and is a significant AIDS-related pathogen. Cryptosporidium parvum is not amenable to long-term laboratory cultivation or classical molecular genetic analysis. The parasite exhibits a complex life cycle, a broad host range, and fundamental mechanisms of gene regulation remain unknown. We have used data from the recently sequenced genome of this organism to uncover clues about gene regulation in C. parvum. We have applied two pattern finding algorithms MEME and AlignACE to identify conserved, over-represented motifs in the 5' upstream regions of genes in C. parvum. To support our findings, we have established comparative real-time -PCR

SUBMITTER: Mullapudi N 

PROVIDER: S-EPMC1779779 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature