Intravital imaging of fluorescent markers and FRET probes by DNA tattooing.

ABSTRACT:

Background

Advances in fluorescence microscopy and mouse transgenesis have made it possible to image molecular events in living animals. However, the generation of transgenic mice is a lengthy process and intravital imaging requires specialized knowledge and equipment. Here, we report a rapid and undemanding intravital imaging method using generally available equipment.

Results

By DNA tattooing we transfect keratinocytes of living mice with DNA encoding fluorescent biosensors. Subsequently, the behavior of individual cells expressing these biosensors can be visualized within hours and using conventional microscopy equipment. Using this "instant transgenic" model in combination with a corrected coordinate system, we followed the in vivo behavior of individual cells expressing

SUBMITTER: Bins AD 

PROVIDER: S-EPMC1779781 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature