The cPLA2 C2alpha domain in solution: structure and dynamics of its Ca2+-activated and cation-free states.

ABSTRACT: Cytosolic phospholipase A2 is involved in several signal transduction pathways where it catalyses release of arachidonic acid from intracellular lipid membranes. Its membrane insertion is facilitated by its independently folding C2alpha domain, which is activated by the binding of two intracellular Ca2+ ions. However, the details of its membrane-insertion mechanism, including its Ca2+-activation mechanism, are not understood. There are several unresolved issues, including the following. There are two experimentally resolved structures of the Ca2+-activated state of its isolated C2alpha domain, one determined using x-ray crystallography and the other determined using NMR spectroscopy, which differ from each other significantly in the spatial region that inserts into the membrane. This by it

SUBMITTER: Varma S 

PROVIDER: S-EPMC1779961 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature