Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing.

ABSTRACT:

Background

RNA interference (RNAi) was originally identified as a biological process in which short double-stranded RNA (dsRNA) suppress the expression of genes complimentary to the dsRNA. This cellular intrinsic gene silencing mechanism has subsequently been developed as a useful tool for studies of gene function. A major strategy for producing small interfering RNA (siRNA) in cultured cells involves the use of siRNA expression vectors in which a RNA polymerase III (Pol III) promoter and transcription stop signal are designed to constitute a functional siRNA expression cassette for production of siRNA. However, most of the available vectors contain only one siRNA expression cassette.

Results

In order to maximize the efficiency and versatility of the vector-based siRNA appro

SUBMITTER: Wang S 

PROVIDER: S-EPMC1780051 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature