High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing.

ABSTRACT: Knowing gene structure is vital to understanding gene function, and accurate genome annotation is essential for understanding cellular function. To this end, we have developed a genome-wide assay for mapping introns in Saccharomyces cerevisiae. Using high-density tiling arrays, we compared wild-type yeast to a mutant deficient for intron degradation. Our method identified 76% of the known introns, confirmed 18 previously predicted introns, and revealed 9 formerly undiscovered introns. Furthermore, we discovered that all 13 meiosis-specific intronic yeast genes undergo regulated splicing, which provides posttranscriptional regulation of the genes involved in yeast cell differentiation. Moreover, we found that approximately 16% of intronic genes in yeast are incompletely spliced during expon

SUBMITTER: Juneau K 

PROVIDER: S-EPMC1780280 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature