Molecular analysis of hepatitis B virus "a" determinant in asymptomatic and symptomatic Mexican carriers.

ABSTRACT:

Background

Hepatitis B virus (HBV) is a small DNA-containing virus with 4 genes, C, S, X and P. The S gene codes for the surface antigen (HBsAg), which contains the "a" determinant, the main region for induction of a protective humoral immune response. To compare the genotype and sequence of the "a" determinant between strains isolated from asymptomatic and symptomatic Mexican HBV carriers.

Results

21 asymptomatic (blood donors) and 12 symptomatic (with clinical signs and with >1 year lamivudine treatment) HBV carriers were studied; all patients were positive for the HBsAg in serum. Viral load, genotypes, and subtypes were determined in plasma. A fragment of the S gene including the "a" determinant was PCR amplified and sequenced to determine genotype, subtype and to identif

SUBMITTER: Ruiz-Tachiquin ME 

PROVIDER: S-EPMC1781063 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature