Bead injection for biomolecular assays: Affinity chromatography enhanced by bead injection spectroscopy.

ABSTRACT: Selective capture of target biomolecules by ligands immobilized on a solid support is a cornerstone of two seemingly unrelated techniques: micro-Affinity Chromatography (microAC) and micro-Bead Injection Spectroscopy (microBIS). This work shows, for the first time, how these techniques can be carried out using the same instrument and how the data obtained this way complement each other, yielding complete information on retention and elution of target biomolecules. Biomolecular association and dissociation were investigated by microAC and microBIS, using computer-controlled programmable flow and the same instrument for automated bead transport, packing of a micro-column, assay of the analyte, and bead disposal. The absorbance of the analyte was monitored within the fiber optic flow cell con

SUBMITTER: Gutzman Y 

PROVIDER: S-EPMC1781929 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature