Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.

ABSTRACT: Tlr elements are a novel family of approximately 30 putative mobile genetic elements that are confined to the germ line micronuclear genome in Tetrahymena thermophila. Thousands of diverse germ line-limited sequences, including the Tlr elements, are specifically eliminated from the differentiating somatic macronucleus. Macronucleus-retained sequences flanking deleted regions are known to contain cis-acting signals that delineate elimination boundaries. It is unclear whether sequences within deleted DNA also play a regulatory role in the elimination process. In the current study, an in vivo DNA rearrangement assay was used to identify internal sequences required in cis for the elimination of Tlr elements. Multiple, nonoverlapping regions from the approximately 23-kb Tlr elements were indepe

SUBMITTER: Wuitschick JD 

PROVIDER: S-EPMC178349 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature