PkaPS: prediction of protein kinase A phosphorylation sites with the simplified kinase-substrate binding model.

ABSTRACT:

Background

Protein kinase A (cAMP-dependent kinase, PKA) is a serine/threonine kinase, for which ca. 150 substrate proteins are known. Based on a refinement of the recognition motif using the available experimental data, we wished to apply the simplified substrate protein binding model for accurate prediction of PKA phosphorylation sites, an approach that was previously successful for the prediction of lipid posttranslational modifications and of the PTS1 peroxisomal translocation signal.

Results

Approximately 20 sequence positions flanking the phosphorylated residue on both sides have been found to be restricted in their sequence variability (region -18...+23 with the site at position 0). The conserved physical pattern can be rationalized in terms of a qualitative binding m

SUBMITTER: Neuberger G 

PROVIDER: S-EPMC1783638 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature