TAMGeS: a Three-array Method for Genotyping of SNPs by a dual-colour approach.

ABSTRACT:

Background

Many of the most effective high-throughput protocols for SNP genotyping employ microarrays. Genotypes are assessed by comparing the signal intensities that derive from the hybridization of different allele-specific probes labelled either by using four fluorescent dyes, one for each base, or by using only two dyes and investigating the polymorphic alleles two by two on separate arrays. The employment of only two dyes makes it possible to use a dual-laser scanner, which has the advantage of being present in every microarray laboratory. However, this protocol may present some drawbacks. To infer all the six possible genotypes it is necessary to compare signals from two arrays, but this comparison not always is successful. A number of systematic errors in the experimental pr

SUBMITTER: Cozza A 

PROVIDER: S-EPMC1783851 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature