Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome.

ABSTRACT:

Background

Several lines of evidence support the existence of novel genes and other transcribed units which have not yet been annotated in the Arabidopsis genome. Two gene prediction programs which make use of comparative genomic analysis, Twinscan and EuGene, have recently been deployed on the Arabidopsis genome. The ability of these programs to make use of sequence data from other species has allowed both Twinscan and EuGene to predict over 1000 genes that are intergenic with respect to the most recent annotation release. A high throughput RACE pipeline was utilized in an attempt to verify the structure and expression of these novel genes.

Results

1,071 un-annotated loci were targeted by RACE, and full length sequence coverage was obtained for 35% of the targeted genes. We

SUBMITTER: Moskal WA 

PROVIDER: S-EPMC1783852 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature