Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran.

ABSTRACT:

Background

Members of Anopheles maculipennis complex are effective malaria vectors in Europe and the Caspian Sea region in northern Iran, where malaria has been re-introduced since 1994. The current study has been designed in order to provide further evidence on the status of species composition and to identify more accurately the members of the maculipennis complex in northern Iran.

Methods

The second internal transcribed spacer of ribosomal DNA (rDNA-ITS2) was sequenced in 28 out of 235 specimens that were collected in the five provinces of East Azerbayjan, Ardebil, Guilan, Mazandaran and Khorassan in Iran.

Results

The length of the ITS2 ranged from 283 to 302 bp with a GC content of 49.33-54.76%. No intra-specific variations were observed. Construction of phylogene

SUBMITTER: Djadid ND 

PROVIDER: S-EPMC1784096 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature