V(D)J recombination in zebrafish: Normal joining products with accumulation of unresolved coding ends and deleted signal ends.

ABSTRACT: V(D)J recombination proceeds from a site-specific cleavage to an imprecise end joining, via generation and resolution of recombination ends. Although rearranged antigen receptor genes isolated from zebrafish (Danio rerio) resemble those made in mammals, differences may arise during evolution from lower to higher vertebrates, in regard to efficiency, fidelity and regulation of this recombination. To elucidate the V(D)J recombination reaction in zebrafish, we characterized recombination ends transiently produced by zebrafish lymphocytes, as well as joining products. Similar to their mammalian counterpart, zebrafish lymphocytes make perfect signal joints and normal coding joints, indicating their competent end resolution machinery. However, recombination ends recovered from the same zebrafish

SUBMITTER: Li Z 

PROVIDER: S-EPMC1785108 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature