A time- and cost-saving method of producing rat polyclonal antibodies.

ABSTRACT: Producing antibodies usually takes more than three months. In the present study, we introduce a faster way of producing polyclonal antibodies based on preparation of the recombinant oligopeptide as antigen followed by immunization of rats. Using this method, we produced antisera against two mouse proteins: ERGIC-53 and c-Kit. An expression vector ligated with a pair of complementary synthetic oligodeoxyribonucleotides encoding the protein was introduced into bacteria, and the recombinant oligopeptide fused with the carrier protein glutathione-S-transferase was purified. Wistar rats were immunized by injecting the emulsified antigen subcutaneously into the hind footpads, followed by a booster injection -after 2 weeks. One week after the booster, the sera were collected and examined for the

SUBMITTER: Wakayama T 

PROVIDER: S-EPMC1790970 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature