Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution II: dynamics.

ABSTRACT:

Background

To accurately describe gene expression and computationally model animal transcriptional networks, it is essential to determine the changing locations of cells in developing embryos.

Results

Using automated image analysis methods, we provide the first quantitative description of temporal changes in morphology and gene expression at cellular resolution in whole embryos, using the Drosophila blastoderm as a model. Analyses based on both fixed and live embryos reveal complex, previously undetected three-dimensional changes in nuclear density patterns caused by nuclear movements prior to gastrulation. Gene expression patterns move, in part, with these changes in morphology, but additional spatial shifts in expression patterns are also seen, supporting a previously prop

SUBMITTER: Keranen SV 

PROVIDER: S-EPMC1794437 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature