Rev-dependent lentiviral expression vector.

ABSTRACT:

Background

HIV-responsive expression vectors are all based on the HIV promoter, the long terminal repeat (LTR). While responsive to an early HIV protein, Tat, the LTR is also responsive to cellular activation states and to the local chromatin activity where the integration has occurred. This can result in high HIV-independent activity, and has restricted the use of LTR-based reporter vectors to cloned cells, where aberrantly high expressing (HIV-negative) cells can be eliminated. Enhancements in specificity would increase opportunities for expression vector use in detection of HIV as well as in experimental gene expression in HIV-infected cells.

Results

We have constructed an expression vector that possesses, in addition to the Tat-responsive LTR, numerous HIV DNA sequences

SUBMITTER: Wu Y 

PROVIDER: S-EPMC1797186 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature